Administration of Lactobacillus plantarum Lp62 to dam rats at the end of delivery and during lactation affects TGF-β1 level and nutritional milk composition, and body weight of pups

Purpose: Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans and exhibited probiotic potential. The influence of oral administration of L. plantarum Lp62 on the growth of rat’s pups; on yield, cytokines and milk composition was studied. Methods: Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans. It was administered daily by gavage to Wistar rats (n = 8), from the 7th day before delivery and for 20 days during lactation, in a concentration of 1.44 × 10 9 CFU/rat. The dam and pups were weighed and milk was collected at 12th and 19th day for determination of protein, triglycerides, cholesterol and lactose by colorimetric assays. TGF-β1 milk levels were analyzed by ELISA. The mammary glands of rats were removed for histological analysis. To detect statistical differences between the groups, tests of mean differences at a significance level of 5% was performed. Results: Supplementation with L. plantarum L62 resulted in significant higher weight of pups (p 0.05). The milk yield was not altered by L. plantarum treatment, but the levels of protein, triglycerides and cholesterol were increased (p 0.05). Levels of TGF-β1 were higher in the milk of L. plantarum treatment (p < 0.05). Conclusions: The treatment of dams at the end of pregnancy and lactation with L. plantarum Lp62 increased nutritional content of milk, probably contributing to the higher weight of the pups. The higher levels of TGF-β1 in the milk, could promote immune benefits to the pups. Further studies in this field are needed to prove the potential use of L. plantarum Lp62 as a probiotic.Fil: Messias, Gladistone Correia. Universidade Federal da Bahia; BrasilFil: Rocha, Ana Marta Novais. Universidade Federal da Bahia; BrasilFil: Santos, Beatriz Manuela Silva. Universidade Federal da Bahia; BrasilFil: Botelho, Ana Marques. Universidade Federal da Bahia; BrasilFil: Silva, Dhaísa Cristhina Alves. Universidade Federal da Bahia; BrasilFil: Porto, Erika Santos. Universidade Federal da Bahia; BrasilFil: dos Anjos, Marina Lima. Universidade Federal da Bahia; BrasilFil: Sousa, Rayra Almeida. Universidade Federal da Bahia; BrasilFil: Silva, Mara Viana. Universidade Federal da Bahia; BrasilFil: da Rocha, Thainara Barros. Universidade Federal da Bahia; BrasilFil: de Melo, Aracely Vieira. Universidade Federal da Bahia; BrasilFil: Carneiro, Manoela Rios Trindade. Universidade Federal da Bahia; BrasilFil: Aguiar, Nayonara Santana. Universidade Federal da Bahia; BrasilFil: Santos, Patrícia Prado. Universidade Federal da Bahia; BrasilFil: de Souza, Erika Pereira. Universidade Federal da Bahia; BrasilFil: Cruz, Mariluze Peixoto. Universidade Federal da Bahia; BrasilFil: Marques, Lucas Miranda. Universidade Federal da Bahia; BrasilFil: Rezende, Raquel Passos. Universidade Federal da Bahia; Brasil. Universidade Estadual de Santa Cruz; BrasilFil: Romano, Carla Cristina. Universidade Federal da Bahia; Brasil. Universidade Estadual de Santa Cruz; BrasilFil: Uetanabaro, Ana Paula. Universidade Estadual de Santa Cruz; BrasilFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Yatsuda, Regiane. Universidade Federal Da Bahia; Brasi