Enhanced IRES activity by the 3'UTR element determines the virulence of FMDV isolates
- García Núñez, María Soledad
- Gismondi, Maria Ines
- König, Guido Alberto
- Berinstein, Analía
- Taboga, Oscar Alberto
- Rieder, Elizabeth
- Martínez Salas, Encarnación
- Carrillo, Elisa Cristina
DOIAbstract
A reverse genetics approach was used to identify viral genetic determinants of the differential virulence displayed by two field foot-and-mouth disease virus (FMDV) strains (A/Arg/00 and A/Arg/01) isolated in Argentina during the 2000-2001 epidemics. A molecular clone of A/Arg/01 strain and viral chimeras containing the S-fragment or the internal ribosome entry site (IRES) of A/Arg/00 in the A/Arg/01 backbone were constructed and characterized. The IRES appeared as a determining factor of the lower level of A/Arg/00 replication in cell culture. High-throughput RNA probing revealed structural differences between both IRESs. Translation experiments using either synthetic viral RNAs ( in vitro) or bicistronic plasmids ( in vivo) showed that th...
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