Aspergillus fischeri mediated biosynthesis of gold nanoparticles and their beneficially comparative effect on normal and cancer cell lines

Background: Along with the intensified use of metal nanoparticles, growing concern of their adverse outcome on human health has also expanded, indicating that this work is an integral part of nanobioscience study. Objective: The aim of this study was to evaluate varied effect of biosynthesized gold nanoparticles (AuNPs) on normal and cancerous mammalian cells. Methods: AuNP synthesized and characterized by different characterization methods, here are produced by specifically isolated Aspergillus species, which is hardly explored in precious scientific findings. These bio-synthesized AuNP are then tested by MTT assay and further confirmed by different fluorescent staining methods. Results: The AuNPs synthesized here are fairly monodispersed and uniform in shape as confirmed by the relatively tall and dominant UV spectroscopy peak at 540 nm and TEM images showing round shaped particles at a magnification of 20 and 50 nm size ranges. The crystalline powder formed peaks at 2θ angles specific for AuNPs verifying their presence and FT-IR validates presence of side groups which help in stabilization of particulates. We have clearly proved that the less toxic nature of these AuNPs on mouse fibroblasts cells and comparatively elevated inhibiting effect on cancer cells at as low as 1 µg/mL concentration of AuNP. Condensation of nucleus and damage of cells seen in fluorescent images also substantiates the results. Conclusion: This conclusion is encouraging to the nanoscientists to study elaborately their system pathways which might be responsible for varied toxicity levels of these AuNPs in mammalian cells and pursue different methods in biomedical applications