Relative functional and optical absorption cross-sections of PSII and other photosynthetic parameters monitored in situ, at a distance with a time resolution of a few seconds, using a prototype light induced fluorescence transient (LIFT) device
- Osmond, B
- Chow, WS
- Wyber, R
- Zavafer, A
- Keller, B
- Pogson, BJ
- Robinson, SA
DOIAbstract
The prototype light-induced fluorescence transient (LIFT) instrument provides continuous, minimally intrusive, high time resolution (∼2s) assessment of photosynthetic performance in terrestrial plants from up to 2m. It induces a chlorophyll fluorescence transient by a series of short flashes in a saturation sequence (180 ∼1s flashlets in <380s) to achieve near-full reduction of the primary acceptor QA, followed by a relaxation sequence (RQA; 90 flashlets at exponentially increasing intervals over ∼30ms) to observe kinetics of QA re-oxidation. When fitted by the fast repetition rate (FRR) model (Kolber et al. 1998) the QA flash of LIFT/FRR gives smaller values for FmQA from dark adapted leaves than FmPAM from pulse amplitude modulated (PAM) ...
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