Detection of Plasmodium falciparum-infected red blood cells by optical stretching

We present the application of a microfluidic optical cell stretcher to measure the elasticity of malaria-infected red blood cells. The measurements confirm an increase in host cell rigidity during the maturation of the parasite Plasmodium falciparum. The device combines the selectivity and sensitivity of single-cell elasticity measurements with a throughput that is higher than conventional single-cell techniques. The method has potential to detect early stages of infection with excellent sensitivity and high speed. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3458919