Add to ORKGTranscriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3\u27 end processing
AbstractRNA interference (RNAi) can be used to study gene function by effecting degradation of the t...
Gene regulation is an integral part of cellular function and key to understanding how genetic materi...
AbstractThe development of a repressible chloroplast gene expression system in Chlamydomonas reinhar...
Chloroplast genes in higher plants frequently synthesize multiple RNA transcripts. Part of this hete...
To determine whether chloroplast RNA polymerase would accurately terminate transcription in vitro, t...
The expression of genes is tightly controlled, predominantly at the point of transcription. RNA poly...
Gene expression analysis is important to understand complex regulatory mechanism and identiWcation ...
Die gegenwärtige Vorstellung von Genexpression in Plastiden leitet sich von der Analyse weniger, ind...
Transcription termination by the RNA polymerase (RNAP) is a fundamental step of gene expression that...
Since its development in 1983 (1), Ribonuclease Protection Assay (RPA) has become a widely employed ...
Gene expression is a multi-step process, which proceeds from DNA through RNA to protein. The tight r...
In Chlamydomonas reinhardtii, the origin for chloroplast DNA replication, Ori A. overlaps the coding...
The real-time PCR assay method was used to quantify the RNA abundance of twenty-eight plastid genes ...
International audienceDuring evolution of photosynthetic organisms, the genetic information provided...
The plant immune system involves detection of pathogens via both cell-surface and intracellular rece...
AbstractRNA interference (RNAi) can be used to study gene function by effecting degradation of the t...
Gene regulation is an integral part of cellular function and key to understanding how genetic materi...
AbstractThe development of a repressible chloroplast gene expression system in Chlamydomonas reinhar...
Chloroplast genes in higher plants frequently synthesize multiple RNA transcripts. Part of this hete...
To determine whether chloroplast RNA polymerase would accurately terminate transcription in vitro, t...
The expression of genes is tightly controlled, predominantly at the point of transcription. RNA poly...
Gene expression analysis is important to understand complex regulatory mechanism and identiWcation ...
Die gegenwärtige Vorstellung von Genexpression in Plastiden leitet sich von der Analyse weniger, ind...
Transcription termination by the RNA polymerase (RNAP) is a fundamental step of gene expression that...
Since its development in 1983 (1), Ribonuclease Protection Assay (RPA) has become a widely employed ...
Gene expression is a multi-step process, which proceeds from DNA through RNA to protein. The tight r...
In Chlamydomonas reinhardtii, the origin for chloroplast DNA replication, Ori A. overlaps the coding...
The real-time PCR assay method was used to quantify the RNA abundance of twenty-eight plastid genes ...
International audienceDuring evolution of photosynthetic organisms, the genetic information provided...
The plant immune system involves detection of pathogens via both cell-surface and intracellular rece...
AbstractRNA interference (RNAi) can be used to study gene function by effecting degradation of the t...
Gene regulation is an integral part of cellular function and key to understanding how genetic materi...
AbstractThe development of a repressible chloroplast gene expression system in Chlamydomonas reinhar...