ISOLATION AND SCREENING OF THERMOPHILIC BACTERIA PRODUCING LIPASE ENZYME IN GEOTHERMAL AREAS OF SIPOHOLON TARUTUNG, NORTH SUMATRA

ABSTRACK Research on the isolation and screening of thermophilic bacteria producing lipase enzymes from the Sipoholon-Tarutung geothermal region has been carried out at the Microbiology Research Laboratory, Department of Biology, AndalasUniversity from December 2018 to March 2019. Bacteria were isolated from hot springs at Sipoholon-Tarutung, North Sumatra. This study aims to obtain thermophilic bacteria and obtain the characterization of thermophilic bacteria. This study used a purposive sampling method based on gradient temperatures in hot springs which ranged from 50°C to 60°C. Bacterial colonies were inoculated using nutrient agar (NA). The results of this study obtained 11 bacterial isolates, of which 7 bacterial isolates showed the lipase enzyme producer. The BTPL-01 isolate originates from pond VI point 1 with a temperature of 55°C, has the highest lipolytic index value of 0.85. BTPL-1 has microscopic characteristics such as gram-positive properties, short basil colony forms, monobacillic structure, positive catalasetest. Keywords: Bacteria Thermophilic, Isolation, Enzyme, Lipase, Lipolyti