Developing synthetic multi-fluorescence techniques in S. cerevisiae for single molecule microscopy

Microscopy has served as a crucial tool for the advancement of biological knowledge, such as discoveries of cellular and subcellular components and mechanisms, molecular structures and function, and protein interactions. Recently, there is an increasing demand for imaging individual cells and molecules, which has driven the development of new microscopy techniques focused on this purpose. Due to technical challenges, these techniques are mostly implemented in vitro. However, there is a push towards in vivo assays, despite potential difficulties. This project aims to exploit the genetic tractability of S. cerevisiae, a simple, eukaryotic model organism, in order to develop fluorescent assays that will improve single molecule microscopy imaging for biological studies in vivo, using dynein motor proteins as a test model