ekstraksi protein dan identifikasi asam amino pada mikroalga chlorella pyrenoidosa dalam mediat ekstrak tauge

Research on microalgae Chlorella pyrenoidosa aims to determine the content of protein and amino acids contained in Chlorella pyrenoidosa.Chlorella pyrenoidosa grow in extract bean sprouts media with urea as nitrogen source and the growth measured at a wavelength of 595nm. Protein extraction using the method of alkali (alkaline extraction) and determination of protein content using the method of Lowry, obtained the protein content of 23,3890 mg / L. Results of the analysis of amino acids obtained 17 kinds of amino acids comprising 7 essential amino acids and 10 non-essential amino acids. The highest amine acid content obtained in 5 types of amino acids Glutamic acid (4.3%), aspartic acid (3.9%), Leucine (3.5%), alanine (3.2%) and Methionine (3.1%