Efficacy of hesperidin (HDN), on apoptotic markers in tissue of normal and myocardial ischemic rats

Myocardial Infarction (MI) results from the interruption of blood supply to a part of the heart causes heart cells to die. In this process, cardiomyocytereach the state of ischemia. Hesperidin (HDN) is naturally occuring flavonoids in citrus and vegetables, have potent antioxidant property. In this study, myocardial ischemia was induced in the experimental rats by subcutaneous injection of Isoproterenol hydrochloride 85 mg/kg b.w., dissolved in saline for two consecutive days. The optimum dose of HDN 200 mg/ kg b.w., was administered to rats post orally for seven days after ISO-induction. In this study, expression of apoptotic markers TNF-α, Fas, Caspase-3, -9, Bax, Bcl-2, Bcl-xL proteins were analyzed by western blot. ISO induced ischemic rats showed up regulation of TNF-α, Fas, Caspase-3, -9 and Bax and all are down regulated after adminstration of HDN. The heart of ischemic rats showed the down regulated expression of Bcl-2 and Bcl-xL. Administration of HDN of ischemic rats, upregulated the expression of Bcl-2 and Bcl-xL expression. Based on this findings HDN conforms the anti apoptotic property. Keywords:  Hesperidin, Myocardial Infarction, Apoptosis, Isoprotereno