THE INFLUENCE OF DIFFRENT CONCENTRATION OF CYTOKININ OF MENTHA PIPERITA

Mentha piperita are important species of the family Lamiaceae. The experiments evaluate the effect of different growth hormons. This study was performed to develop a protocol by standardizing the different hormonal concentrations . Regeneration of Mentha piperita in vitro and in vivo was obtained by treating seed of Mentha with different concentration and combinations of auxin and cytokinin for the formation of plant s. Sterilization of the seed for in vitro was obtained by treating with etanol 70% for 15 sec with 2-3 drops of Tween-20 (a detergent)for in vivo used CCloroxTM solution (5.25 % NaOCl ) and then rinsed thrice with double distilled water. Fully developed plantlets were successfully transferred to suitable growing media for acclimatization and there further growth and development. Experiment was performed to study the effect of different concentration of Hormons BAP(1,0mg/l) ,KIN(1,5mg/l),NAA(1,0mg/l) on the germination and growth of Mentha piperita explants. The parameters under study were number of days to the effect on germination in vitro and in vivo and growth of the plant . Increase of the hormone doses resulted in reduced growth