Screening Antibacterial Activity of Combination of Secang Extract (Caesalpinia sappan) and Clove oil (Syzigium aromaticum) Againts Propionibacterium acnes by Diffusion Method

Propionobacterium acnes are common bacteria that cause an acne. It is populate in the skin and induce the inflammatory phase of acne. The methanolic extract of secang (Caesalpinia sappan) were proven that have an antiacne potency and the clove oil (Syzigium aromaticum) could inhibit the growth of propionibacterium acnes by disk diffusion method. The aim of this study is to investigated the potency of combination of both sample to inhibit Propionibacterium acnes by well diffusion method. The results showed that the antibacterial assay of the combination of methanolic extract secang and clove oil (1:1 v/v) was more effective than single samples. The combination could inhibit the growth of propionibacterium acnes with inhibition zone value is 19.3 mm. The inhibition zone value of methanolic extract of secang (8 mg/mL) and clove oil (20 µl/mL) respectively is 17.6 mm and 10.6 mm. This combination has the potential to be developed into an antiacne product so it requires further research to find the right formula combination from both samples to enhance their activity