Efek Dekokta Daun Pulutan (Urena lobata) Terhadap Kadar Hemoglobin dan Persentase Sel Darah Merah Abnormal Ikan Zebra (Danio rerio) Dewasa yang Dipapar Malathion Secara Kronik

Introduction: Malathion is a type of organofostat pesticide that go through biotransformation in the body into malaoxon and free radical compounds. Malaoxon works as an inhibitor of acetylcholinesterase and free radicals so it can cause cholinergic crisis and affect the structure and function of red blood cells. Decoction of pulutan leaf has potential as an antioxidant, anti-inflammatory, and prevent bleeding. This study aims to prove the effect of pulutan decoction leaves on hemoglobin levels and the percentage of zebrafish red blood cells exposed to malathion chronically.Method: Experimental laboratory in vivo with the control group post test design only. Fish are divided into 2 control groups and 3 treatment groups with 5 ppm malathion dosage and 125 mg/L, 250 mg/L and 500 mg/L of pulutan decoction leaves. Hb levels are seen with comersial Hb meters. The description of red blood cells observed by making blood smear with giemsa staining and then examined in a binocular microscope with 400X magnification and percentage. Data analysis using one-way ANOVA and LSD with a significance level of p