Uji Aktivitas Antibakteri Ekstrak Etanol Kulit Batang Delima (Punica granatum. L)Terhadap Bakteri Staphylococus aureus ATCC 6538 Tahun 2016

Background: Pomegranate bark (Punica granatum, L) contains ellagitannin, triterpenoid, alkaloids consisting of pelletierine, methylpelletieren, pseudopelletierine, flavonoids, and saponins. Substances that are able to inhibit bacterial growth are saponins and flavonoids. Objective: to determine the antibacterial activity of ethanol extract of pomegranate bark (Punicagranatum, L) on the Staphylococcus aureus bacteria. Method: The manufacture of pomegranate bark extract was carried out using the Soxlhetasi method, after extracting the antibacterial activity was carried out by using a cylinder diffusion method. Result: Ethanol extract of pomegranate bark (Punica granatum, L) has antibacterial activity against Staphylococcus aureus bacteria at a concentration of 10% b / v, 25% b / v, 50% b / v and 75% b / v with each inhibition zone diameter -as equal to 18.70 mm, 21.00 mm, 22.30 mm and 26.20 mm. Stastically, ethanol extract of pomegranate bark (Punica granatum, L) has significant antibacterial power with p = 0.001 (p <0.05). Conclusion: Ethanol extract of pomegranate bark (Punica granatum, L) has antibacterial activity against Staphylococcus aureus bacteria with very strong concentration is a concentration of 25% b / v, 50% b / v and 75% b / v, because the inhibition zone is> 20 mm.Latar Belakang: Kulit batang delima (Punica granatum, L) mengandung ellagitannin, triterpenoid, alkaloid yang terdiri dari pelletierine, methylpelletieren, pseudopelletierine, flavonoid dan saponin. Zat yang mampu menghambat pertumbuhan bakteri adalah saponin dan flavonoid. Tujuan: untuk mengetahui aktivitas antibakteri ekstrak etanol kulit batang delima (Punicagranatum, L) terhadap bakteri Staphylococcus aureus. Metode: Pembuatan ekstrak kulit batang delima ini dilakukan menggunakan metode soxlhetasi, setelah ekstrak didapat diklakukan pengujian aktivitas antibakteri dengan metode difusi menggunakan silinder. Hasil:Ekstrak etanol kulit batang delima (Punica granatum, L) mempunyai aktivitas antibakteri terhadap bakteri Staphylococcus aureus pada konsentrasi 10% b/v, 25% b/v, 50% b/v dan 75% b/v dengan diameter zona hambat masing-masing sebesar 18.70 mm, 21.00 mm, 22.30 mm dan 26.20 mm. Secara stastik, ekstrak etanol kulit batang delima (Punica granatum, L) mempunyai daya antibakteri yang bermakna dengan p = 0,001 (p < 0,05). Kesimpulan: Ekstrak etanol kulit batang delima (Punica granatum, L) mempunyai aktivitas antibakteri terhadap bakteri Staphylococcus aureusdengan konsentrasi sangat kuat adalah konsentrasi 25% b/v, 50% b/v dan 75% b/v, karena zona hambatnya > 20 mm