Investigating the Negative Regulation of Iqg1 in Budding Yeast Cytokinesis

Cytokinesis is the separation of cells and is the final step involved in division of the cell. In budding yeast (S. cerevisiae) the protein Iqg1 helps regulate the actin cytoskeleton involved in the contraction ring necessary for cytokinesis. Iqg1 is similar to the IQGAP1 found in humans, which can be more active in tumor cells. The experiment being conducted involves the negatively regulated interaction of Iqg1 with GTPase Tem1 necessary for contraction of the myosin ring. It is hypothesized that the proteins Bfa1 and Bub2 required for the spindle checkpoint regulates the Iqg1-Tem1 interaction. To test this, cells without Bfa1and Bub2 or overexpression of these proteins will be studied to establish what effects occur on the Iqg1-Tem1 co-immunoprecipitation