Add to ORKGS-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program
Background/Aims: NO and reactive nitrogen species (RNS) are thought to be physiologically important ...
In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxi...
The ability of a series of metal oxide nanoparticles (20 - 60 nm) to alter muscarinic receptor signa...
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when intro...
The effect of NO on calcium oscillations elicited by low concentrations of a muscarinic agonist in C...
Oscillation of intracellular calcium concentration is a stable phenomenon that affects cellular func...
Nitric oxide (NO) production and Ca2+ homeostasis are key determinants for the control of many cell ...
As the first line of host defense, neutrophils are stimulated by pro-inflammatory cytokines from res...
AbstractThe actions of the nitric oxide (NO) donors 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3 met...
AbstractThe effects of nitric oxide on the activities of thapsigargin-sensitive sarcoplasmic reticul...
Neuronal nitric oxide synthase (nNOS) is a calcium (Ca2+)/calmodulin (CaM) dependent\ud enzyme that ...
Store-operated Ca2+ entry (SOCE) plays an important role in the contraction and proliferation of pul...
Cell-permeable biphenols, such as honokiol, present in herbal teas, inhibit autonomic responses. The...
In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxi...
Nitric oxide (NO) plays an important role in many physiological and pathophysiological processes in ...
Background/Aims: NO and reactive nitrogen species (RNS) are thought to be physiologically important ...
In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxi...
The ability of a series of metal oxide nanoparticles (20 - 60 nm) to alter muscarinic receptor signa...
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when intro...
The effect of NO on calcium oscillations elicited by low concentrations of a muscarinic agonist in C...
Oscillation of intracellular calcium concentration is a stable phenomenon that affects cellular func...
Nitric oxide (NO) production and Ca2+ homeostasis are key determinants for the control of many cell ...
As the first line of host defense, neutrophils are stimulated by pro-inflammatory cytokines from res...
AbstractThe actions of the nitric oxide (NO) donors 1-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3 met...
AbstractThe effects of nitric oxide on the activities of thapsigargin-sensitive sarcoplasmic reticul...
Neuronal nitric oxide synthase (nNOS) is a calcium (Ca2+)/calmodulin (CaM) dependent\ud enzyme that ...
Store-operated Ca2+ entry (SOCE) plays an important role in the contraction and proliferation of pul...
Cell-permeable biphenols, such as honokiol, present in herbal teas, inhibit autonomic responses. The...
In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxi...
Nitric oxide (NO) plays an important role in many physiological and pathophysiological processes in ...
Background/Aims: NO and reactive nitrogen species (RNS) are thought to be physiologically important ...
In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxi...
The ability of a series of metal oxide nanoparticles (20 - 60 nm) to alter muscarinic receptor signa...