Two-stage Course Embedded Determination of Caffeine and Related Compounds by HPLC in caffeine containing Beverages

During FY 13-14, we have successfully designed and implemented a two-stage project both qualitatively and quantitatively on analyzing caffeine in caffeine-containing products. Our newly acquired High Performance Liquid Chromatography (HPLC) system is used at both stages. The first stage is finished in two consecutive semesters in Chem3000K (Analytical Chemistry) course, mainly focusing on quantitative analysis with a well-developed HPLC method. The second stage of the project is adapted into STEC4500 research as a pilot project for future Chem4100K (Instrumental Chemistry) course, which involves qualitative analysis of caffeine, sugars, and preservative. Variety of sample matrix was tested for both stages including soft drinks and energy drinks. Assessment data shows majority of participant appreciate the research (STEM) opportunities