The Activation of Mature Dendritic Cells Using Nanoparticle Drug Delivery System

Background: Nanocarriers are a part of the highly innovation of nanotechnology engineering. They are generallyeffect, nanoparticle clearance by mononuclear phagocyte systems and desirable nanoparticle characteristics for cancer submissions. Dendritic Cells, (DCs) are the most influential Antigen Presenting Cells (APC) that contributes to the induction of the central and peripheral immunological stimulation at mature status. Dendritic Cells can be generated from bone marrow cell culture supplemented with GM-CSF and IL-4. CpG is capable to stimulate DCs through binging with TLR-9. CpG coating NPs allows for optimal use of the delivery system to dendritic cell activation thus induces immune stimulation, which has potential application for immunotherapies of cancer. Purpose: To determine how Tumor antigen encapsulated Nanoparticles coated with CpG ligand (CpG-NP-Tag) influence maturation of dendritic cells. Methods: Bone marrow cells were isolated and cultured for 5 days in supplement with GM-CSF and IL-4 to generate DCs. CpG-tumor antigen-encapsulated Nanoparticles were loaded to DCs culture for two days. CD80 and CD86 expression, the maturation marker of DCs, were evaluated by flow cytometry. Results: CpG delivered by nanoparticle along with tumor antigen increases maturation of DCs. Conclusion: CpG-NP-Tag NPs help in the maturation of BMDCs which could further build a platform to build a cell mediated adaptive immune response. Taken together, these mechanisms may lead to innovative ways of dendritic Nano-carrier usage and its effectiveness in cancer immunotherapy