Determination of succinic acid in fermentation broth using thin layer scanning technique

基于CuSO4与发酵液中琥珀酸的显色反应,在硅胶板上不经层析展开使用薄层扫描定量分析琥珀酸含量。在0～12 g/L范围内,测定波长680 nm,参比波长500 nm条件下,琥珀酸浓度与显色斑吸光度呈良好线性关系。方法检出限(3 S/k)为0.028 mg/L,回收率94.80%～96.12%,相对标准偏差≤3.5%(n=5)。发酵液中副产物的干扰率≤5.60%。本研究可为实验室研究或工业生产提供一种简便高效的琥珀酸定量检测方法。Based on color reaction between copper sulfate and succinic acid in fermentation broth,succinic acid concentration was determined directly by using thin layer scanning without chromatography.Linear relationship between the value of absorbance and the succinic acid concentration was obtained in the range of 0-12 g/L,using 680 nm as measurement wavelength and 500 nm as reference wavelength for scanning.The method detection limit（3 S/k） was 0.028 mg/L with the recoveries of 94.8%～96.1%,and the RSD（n=5） was lower than 3.5%.The interference rate of byproducts was lower than 5.60%.The paper provided an easy but efficient method for the determination of succinic acid in lab study or industry production.国家民委科研项目(10GX07);广西科学基金(2011GXNSFC018015);广西教育厅科研项目(201012MS072)资