New species emergence via recombination among isolates of the Brazilian tomato infecting Begomovirus complex

A seqüência de nucleotídeos parcial de cinco isolados de Begomovirus foi determinada do DNA-A, correspendente à região intergênica e à porção 5' do gene associado à replicação e da capa protéica. O isolado DFM apresentou identidade de 95% com Tomato mottle leaf curl virus (TMoLCV); os isolados 34, PA-05 e Ta4 foram 88% idênticos ao Tomato yellow vein streak virus (ToYVSV); e o isolado DF-BR3 mostrou 77% de identidade com TMoLCV. Análise de recombinação indicou que o isolado DF-BR3 seria uma quimera e evidencia que um complexo de espécies de begomovírus bipartidos está em formação no Brasil.Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with Tomato mottle leaf curl virus (TMoLCV), isolates 34, PA-05, and Ta4 were 88% identical to Tomato yellow vein streak virus and isolate DF-BR3 shared 77% identity with TMoLCV. Recombination analysis indicated that isolate DF-BR3 was a chimaera, and it provided evidence that there is a complex and actively recombining population of tomato infecting begomoviruses in Brazil