АМПЕРОМЕТРИЧЕСКИЕ МОНОАМИНОКСИДАЗНЫЕ БИОСЕНСОРЫ НА ОСНОВЕ ГРАФИТОВЫХ ЭЛЕКТРОДОВ И ОКСИДА ГРАФЕНА КАК МОДИФИКАТОРА ПОВЕРХНОСТИ ДЛЯ ОПРЕДЕЛЕНИЯ НЕКОТОРЫХ АНТИДЕПРЕССАНТОВ Э.П.Медянцева, Д.В.Брусницын, Р.М.Варламова, Р.Р.Ситдикова, А.Н.Галявина

Novel monoamine oxidase amperometric biosensors based on screen-printed graphite electrodes modified with nanostructured material graphene oxide (GO) was developed for the determination of antidepressants («Melipraminum», «Coaxil» and «Fenazepam»).Response of the biosensor created is based on a combination of monoamine oxidase biochemical action towards biogenic amines (dopamine, serotonin) and electrochemical oxidation of hydrogen peroxide (a product of the enzymatic reaction) as well as the inhibition effect of the studied antidepressants of the immobilized enzyme. Тhe analytic signal is hydrogen peroxide oxidation current at potentials 0.7-0.75 V.The usage of chitosan acetate solution as a dispersant for carbon nanotubes and graphene oxide provides a more pronounced analytical biosensor signals compared to suspension in DMF. The antidepressants under investigation were determined within the wide range concentration of 1·10-4 - 1·10-8 M. The analytical capabilities of the GO-based biosensor with biosensor modified with carbon nanotubes in chitosan have been compared. GO-modified biosensor had certain advantages over biosensors modified with carbon nanotubes, in particular the higher sensitivity coefficient and lower detection limit.The application of acid-base titration as a reference method for determination on example of imipramine has shown the absence of systematic error in measurements using the developed biosensors.The biosensors developed can be applied for the control for residual amounts of drugs in biological fluids (urine) at 9·10-9 M as well as contents of active substance in pharmaceutical dosage forms.Keywords: biosensor, graphene oxide, carbon nanotubes, chitosan, antidepressants, monoamine oxidase, biological fluid (Russian)DOI: http://dx.doi.org/10.15826/analitika.2014.18.4.011 E.P. Medyantseva, D.V. Brusnitsyn, R.M. Varlamova, R.R. Sitdikova, A.N. Galiavina, G.K. 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Сопоставлены аналитические возможности предлагаемых биосенсоров c биосенсорами на основе электродов, модифицированных углеродными нанотрубками в хитозане и ДМФА. Предложенные биосенсоры можно использовать для контроля как остаточных количеств лекарственных препаратов в биологических жидкостях (урина) на уровне 9·10-9 М, так и лекарственного вещества в лекарственных формах.Ключевые слова: биосенсор, оксид графена, углеродные нанотрубки, хитозан, антидепрессанты, моноаминоксидаза, биологическая жидкостьDOI: http://dx.doi.org/10.15826/analitika.2014.18.4.011 ЛИТЕРАТУРА1. Simultaneous determination of new-generation antidepressants in plasma by gas chromatography–mass spectrometry / E. Pietracci [et al.] // Forensic Toxicol. 2013. V. 31. P. 124-132.2. Fernandez-Navarro J. J., Ruiz-Angel M. J., Garcıa-Alvarez-Coque M. C. Reversed-phase liquid chromatography without organic solvent for determination of tricyclic antidepressants // J. Sep. Sci. 2012. V. 35. P. 1303-1309.3. 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