Recovery of «Phaemoniella chlamydospora» and «Phaeoacremonium inflatipes» from Soil and Grapevine Tissues

Rose Bengal Chloramphenicol Agar (RBCA) appears to be a suitable media for isolating Phaeoacremonium spp. and Phaeomoniella chlamydospora from soil, spore traps and plant tissue. Using the soil-plate method, populations of these organisms were recovered from the soil and surfaces of plant tissue from many different regions of California. In addition, in a few vineyard sites these fungi were recovered from dried plant sap, which had oozed from grapevine girdling wounds and from standing water under grapevine drip systems. RBCA, along with a filtering system, is a useful tool in determining the presence of Petri disease pathogens in vineyard soils, water, and plant tissues. This research presents the first report of the recovery of Phaeoacremonium inflatipes from soil and standing water under grapevines