Polymerase Chain Reaction (PCR)

Overview The polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes and its basic requirements for success. Examples of interpreting results are given. PCR’s strengths, weaknesses, and applications to plant biotechnology are explained. Objectives At the completion of this PCR lesson, learners will be able to: Describe what natural cellular process PCR mimics. List the 5 chemical components of a PCR reaction and describe their roles. List the functions of the 3 temperature cycles which are repeated during a PCR reaction. Describe the process of observing results and interpreting results of an experiment. Explain both the strengths and weaknesses of PCR. List possible uses of PCR in plant biotechnology. Modules: Lesson home Introduction for PCR The Discovery of PCR Chemical Components of PCR Temperature Cycles Visualizing the Results An Actual Gel Picture Strengths of PCR Weaknesses of PCR Plant Diagnostics Applications Summary References Glossar