Functional responses and adaptation of mesophilic microbial communities to psychrophilic anaerobic digestion
- Gunnigle, Eoin
- Nielsen, Jeppe L.
- Fuszard, Matthew
- Botting, Catherine H.
- Sheahan, Jerome
- O'Flaherty, Vincent
- Abram, Florence
DOIAbstract
Psychrophilic (<20°C) anaerobic digestion (AD) represents an attractive alternative to mesophilic wastewater treatment. In order to investigate the AD microbiome response to temperature change, with particular emphasis on methanogenic archaea, duplicate laboratory-scale AD bioreactors were operated at 37°C followed by a temperature drop to 15°C. A volatile fatty acid-based wastewater (composed of propionic acid, butyric acid, acetic acid and ethanol) was used to provide substrates representing the later stages of AD. Community structure was monitored using 16S rRNA gene clone libraries, as well as DNA and cDNA-based DGGE analysis, while the abundance of relevant methanogens was followed using qPCR. In addition, metaproteomics, microautoradi...
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