Physical Properties of Bovine Serum Albumin Microspheres Using HPC-L and Hypromellose 606 Polymer

The aim of this study was to investigate thepreparation of microspheres as potential proteincarriers. Bovine serum albumin (BSA) was chosen as a hydrophilic model protein to beencapsulated within hydroxypropyl cellulose low viscosity (HPC-L) and hydroxypropyl methylcellulose 606 (Hypromellose 606) microspheres using spray drying method, which was calledby L and M formula respectively. Both polymers have two different concentrations, low (F1Land F1M) and high concentration (F2L and F2M). The microspheres were evaluated tomorphology, particle size, entrapment efficiency (EE), protein content, yield, and FTIR. Thespray drying method produced rough surfaced microspheres with small particle size rangingfrom 1.18 ± 0.07 µm for F1L to 1.70 ± 0.05 µm for F2M. BSA microspheres had a high EE%ranging from 75.84 ± 2.75% for F1L to 99.29 ± 0.56% for F2L, and highest protein contentwas produced by microspheres with HPC-L polymer at low concentration (F1L) 2.51 ± 0.06%.The FTIR spectra of protein alone and in micropsheres with polymers did not show any shiftin major peak, which indicated no protein-polymer interaction. The results demonstratedthis spray dried microspheres can be explored for potential protein carrier