Composition research of phenolic compounds in old apple cultivars of Lithuania

The aim of the research – to investigate quality and quantity of phenolic compounds in old apple cultivars of Lithuania. 'Antaninis', 'Jono pepinas', 'Lietuvos pepinas', 'Biržuvėnų žieminis', 'Koštelė', 'Sierinka', 'Golden Russet') The objectives of the research: 1. To determinate the variation of the quantitative composition of phenolic compounds of the old apple cultivars by UV–VIS spectrophotometry. 2. To determinate the qualitative and quantitative composition of phenolic compounds in samples of apple fruits by HPLC methodology. 3. To determinate the antiradical activity of ethanol extracts of old varieties of apples in vitro. 4. To determinate the reduction activity of ethanol extracts of old varieties of apples in vitro. Object and methods: Apple fruits samples of cultivars were collected in 2017 grown in Lithuania. The total content of phenolic compounds in apples samples was performed using UV–VIS spectrophotometric method. Antioxidant activity was assessed by DPPH, ABTS, FRAP and TFPH spectrophotometric methods