Qualitative and quantitative composition variability of phenolic compounds in American cranberry (Vaccinium macrocarpon Aiton) fruits grown in Lithuania

The object and methods of the research: the samples of fruits of cranberry cultivars, grown in Lithuania ('Baiwfay', 'Bergman', 'Bain', 'Drever', 'Holliston', 'Pilgrim', 'Searles', 'Woolman'), qualitative and quantitative composition variability was investigated, using HPLC and UHPLC methods. The aim of the research: to determine the qualitative and quantitative composition of phenolic compounds of fruits of cranberry, which was grow in Lithuania and to evaluate the antioxidant activity in vitro of their extracts. The objectives of the research: to determine the extraction conditions of anthocyanins from the samples of fruits of American cranberry. To determine the quantitative composition of phenolic compounds in different cultivars of American cranberry. To determine the antioxidant activity in vitro in the samples of different cranberry cultivars. To determine the qualitative and quantitative composition of phenolic compounds in different cultivar samples of American cranberry fruits by HPLC methodology. To evaluate correlation between cranberry fruit extract containing quantitative composition of total phenolic compounds and the antioxidant activity in vitro. To determine the qualitative and quantitative composition in different cultivar samples of American cranberry fruits by UHPLC methodology. The results and conclusions of the research: the best extraction conditions for anthocianins were determined to be when extraction time is 40 min, ethanol concentration is 70%, ultrasovic bath power is 452W. The highest determined total amount of: anthocianins was 8,54±0,04 mg/g (p<0,05), flavonoids 5,34±0,026mg RE/g (p<0,05), phenolic compounds 18,06±0,15mg/g (p<0,05), proanthocianidins 10,55±0,26 mg EE/g (p