Analysis of food supplements containing glucosamine using chromatography methods

Aim of the research: qualitative and quantitative analysis of glucosamine in food supplements available in Lithuanian market. Tasks of research: chose best analysis methods for experiments; validate the methods chosen for assessment of glucosamine; perform qualitative and quantitative analysis of glucosamine; compere experiment results with declared amounts by manufacturer. Object of the research: food supplements containing glucosamine available in Lithuanian market. Methods: for qualitative and quantitative analysis HPLC and TLC was used. Results: HPLC and TLC showed that in all food supplements chosen for experiment there was traces of glucosamine. Glucosamine amount determined in food supplements ranges from 77,0 % to 94.5 % of amount declared by manufacturer. Conclusions: traces of glucosamine was found in all five food supplements; quantitative analysis showed that amount of glucosamine in food supplements was: No.1 709mg; No.2 798mg; No.3 462mg; No.4 498mg; No.5 426mg; when comparing experiment results with manufacturers declared amount it was found that largest deviation was in sample No. 3 which was 23 % and the lowest deviation was in sample No. 1 that was 5.5 %. Recommendation: for the further analysis of food supplements with glucosamine use of HPLC Waters 2424 ELS detector and BGA Li Chrospher 100 Diol column is advised