Determination of plant hormone-like substances of the japanese knotweed (fallopia japonica) and canadian goldenrod (solidago canadensis) with bioassay (triticum and avena assay)

Japonski dresnik (Fallopia japonica) in kanadska zlata rozga (Solidago canadensis) sta v Sloveniji pogosti invazivni tujerodni vrsti. V naši raziskavi smo poskušali z biotesti na rastlinske hormonom podobne substance določiti hormonski status obeh vrst. Biotesti so enostavna, cenovno ugodna metoda za določanje rastlinskih hormonov in njim sorodnih substanc. Uporabili smo Avena (ovseni) biotest za avksinom podobne substance in Triticum (pšenični) biotest za citokininom podobne substance. V proučevanih rastlinah smo v različnih stadijih rasti našli med 17 μg in 332 μg avksinov na gram suhe mase rastlin, tako v sušenem kot v liofiliziranem rastlinskem materialu. Citokininov z uporabljenimi biotesti nismo zaznali. Liofiliziran material je v primerjavi s sušenim vseboval nekoliko več rastlinskim hormonom podobnih substanc.The japanese knotweed (Fallopia japonica) and canadian goldenrod (Solidago canadensis) are common invasive species in Slovenia that spread very quickly. In our study, we tried to determine the hormonal status of both invasive species with bioassay for plant hormone-like substances. Bioassays are a simple, affordable method for determining plant hormones and related substances. We used Avena (oat) bioassay for auxin-like substances and Triticum (wheat) bioassay for cytokinin-like substances. In studied invasive species, we found between 17 μg and 332 μg of auxins per gram of dried or lyophilized mass of invasive plants at different development stages. No cytokinins were observed with bioassays. The lyophilized material contained slightly more hormone-like substances than dried material