Chemical study on piper betel stems / Nursyahirah Syahidah Baharim

This study is aimed to identify the chemical constituent of Piper betel stems. This sample was collected from Bukit Gantang, Perak. The species of Piper betel is in Piperaceae family. The objective of this study is to extract the Piper betel stems and isolate it to identify the active chemical compound. Piper betel was extracted by using hexane, chloroform and ethanol solvent. This solvent was based on polarity, started with non-polar to polar solvent. The percentage yield for hexane, chloroform and ethanol crude extract are 3.60 %, 2.36 % and 1.27 % respectively. TLC for these extract showed ethanol extract give the best spot, solvent system hexane:ethyl acetate. Column chromatography was done to isolate the mixture compound from ethanol extract with mobile phase was hexane:ethyl acetate and ethyl acetate:methanol. The fractions collected was 53 vials. Fractions 17 (PB1) and 23 (PB2) was selected for further analysis based on their TLC profiling. For characterization of chemical compound, UV-Vis showed maximum wavelength λ=210 nm and 225. FTIR showed several absorption and GCMS showed molecular weight m/z = 280 and 282. Based on characterization analysis, the major compound found in Piper betel stems are was probably identified as oleic acid (PB1) and linoleic acid (PB2)