The hypnotic effect of benzoylthioutea derivative (2.4 dichlorobenzoylthiourea) in mice

The structure modification of benzoylthiourea by adding Cl atoms at the position of C2 and C4 may result in 2,4-dichlorobenzoylthiourea. With the addition of two Cl atoms on the benzoiltiourea aromatic ring, log P value may increase to 2.24 and the synthesized compound is expected to have better biological activity as a central nervous system deppressant (hypnotic effect) than the lead compound. This compound has similar structure to barbiturates. In this study, the test of hypnotic effect was performed on white male mice by observing the mice sleep duration extension. The test animals were divided into 7 groups: the control group was given 0.5% Na-CMC suspension; the standard were given 125 mg/kg of Phenobarbital Na; while treatment group I, II, Ill, IV, and V were orally given 15 mg/kgBW, 30 mg/kgBW, 45 mg/kgBW, 60 mg/kgBW, and 75 mg/kgBW of suspensions of 2,4-dichlorobenzoylthiourea respectively, with the Time Peak Effect (TPE) : 30 minutes. The results showed that the average sleep duration of the control group, standards, treatment I, II, Ill, IV, and V were 0 minute, 33.37 minutes, 87.14 minutes, 178.67 minutes, 232.91 minutes, 272.60 minutes, and 304.66 minutes respectively. The ANOVA statistical results with a=0.05 showed significant differences between the groups and it can be concluded that 2,4- dichlorobenzoylthiourea has stronger hypnotic effect than Phenobarbital Na in white male mice