Sensitivity testing of vankomycin resistant and vancomycin sensitive enteroccoci on selected biocides

Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Healthcare bioanalytics Candidate: Martina Malíková Thesis supervisor: PharmDr. Ondřej Janďourek, Ph.D. Consultant: RNDr. Irena Hanovcová, CSc. Title of bachelor thesis: Testing the sensitivity of vancomycin-resistant and vancomycin-sensitive enterococci on selected biocides Vancomycin resistant enterococci (VRE) are one of the most common causes of nosocomial infections. The aim of this work was to determine the sensitivity of vancomycin-resistant and vancomycin-sensitive enterococci to selected biocides, which are part of disinfection programs in healthcare facilities. When testing the effectiveness of disinfectants, we followed the Czech technical standard ČSN EN 1040 - Chemical disinfectants and antiseptics - Quantitative suspension test to determine the basic bactericidal effect of chemical disinfectants and antiseptics - Test method and requirements (Phase 1). We used a dilution method with a neutralizer. We tested a total of 10 Enterococcus faecium strains. Four out of the 13 biocides tested were completely effective. These were agents with a higher concentration of aldehydes, with peracetic acid and active chlorine. We observed different efficacy in individual strains for..