Effect of cell passage time on the electrotransfection efficiency

An efficient gene transfer technique leads to subsequently improve the gene therapy protocols. Since last few decades, an electroporation has been a better alternative compared to the viral methods to be employed in in vitro and in vivo protocols. The mechanism is based on the formation of reversible pores in the cell by applying the short duration electric pulses in order to ease the injection of molecules. However, an optimal transfection efficiency without losing a significant number of cells is still a big concern for the researchers. Therefore, most of the research work has been based on the improvement of transfection efficiency. In this work, the effect of cell passage time on the transfection efficiency of electroporation has been analyzed by considering the different plasmid concentrations. We observed the significant increase in the transfection efficiency for all plasmid concentrations with 24 h cell passage time in comparison to 48 h cell passage time without a significant change in cell viability. These results are very useful in order to conduct future research for the improvement of elctrotransfection efficiencyBiologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta