Population genetic structure of wild boar in African Swine Fever infected area of Lithuania during 2014-2017

The wild boar is one of the most common mammals in Lithuania, which is currently facing a major challenge - African swine fever. The purpose of this study was to characterize a population of wild boar (Sus scrofa) in Lithuania, using microsatellite analysis. The analysis involved African Swine Fever infected animals during 2014 – 2017. The 16 microsatellite locus analysis were performed using 9 – plex and 7 – plex PCR amplification and fragment analysis with ABI3100 sequencer. Samples were collected from various regions of Lithuania: Vilnius, Kaunas, Šiauliai, Panevėžys and Alytus and are divided into four groups by year The study material blood or tissue collected from 215 hunted or dead animals. The average value of the observed heterozygosity was Ho = 0.525, the average value of expected heterozygosity He = 0.754, observed overall within – population inbreeding estimate FIS = 0.316Biologijos katedraGamtos mokslų fakultetasNacionalinis maisto ir veterinarijos rizikos vertinimo institutasVytauto Didžiojo universiteta