Microsatellite analysis of wild boar infected with African swine fever

Currently, one of the biggest problems in Lithuania is the spreading African swine fever - the one of the most serious disease of wild boar and domestic pigs. Many infected, dead wild boars are found in the infected area, but in this situation, the subspecies of wild boars are almost not decreasing or decreasing marginally. Perhaps one of the reasons is that certain individuals or their groups are resistant to the virus. The purpose - investigation of the genetic variations of wild boar sampled in areas infected with African swine fever. First portion of analysis was genetic investigation of 96 ASF positive samples during 2014 – 2016 using Microsatellite analysis method. 16 microsatellite locus analysis were performed using 9-pelx and 7 – plex PCR amplification and fragment analysis with ABI3100 sequencer. The samples were divided in to 5 groups representing infected district of Lithuania: Vilniaus, Kauno, Utenos, Panevezio and Alytaus district. Among the analysed 16 microsatellites markers the highest variation is in S0005 locus and in locus SW24. The lowest variation is observed in locus S0068 and S0355Biologijos katedraGamtos mokslų fakultetasNacionalinis maisto ir veterinarijos rizikos vertinimo institutasVytauto Didžiojo universiteta