Microbiological activity of blended essential oil of medicinal plants Zingibe officinale L. and Cymbopogon citratus L

Essential oils of Zingibe officinale L. and Cymbopogon citratus L. were obtained from a commercial source. They were blended at different ratios (from 0.005% to 50.0%). For microbiological activity of essential oils blends two Gram positive (Staphylococcus aureus DSM-No. 799, Enterococcus faecium DSM-No. 2918) and three Gram negative (Pseudomonas aeruginosa DSM-No. 939, Escherichia coli, Proteus mirabilis DSM-No. 788) bacteria species as well as yeast Candida albicans DSM-No. 1386 were used. Microorganisms species were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Germany). These five germs are commonly used for the evaluation of disinfectant agents. Quantitative and qualitative analyses of essential oils were carried out using gas chromatograph with mass spectrometric detector (Shimadzu GC-2010 Japan) equipped with the capillary column. The Zingibe officinale L. (major essential oils components zingiberene and bisabolene) combined with Cymbopogon citratus L. (major components neral, geraniol) -exhibited the most potent activity against microorganisms. Our result suggested that the mixed functional groups of the major component may be justified for the assessment of their bioactivity. Cymbopogon citratus L. and Zingibe officinale L. presented antimicrobial activity for St. aureus, P. mirabilis, C. albicans, E. coli (MIC=0.05%). As used concentration Cymbopogon citratus L. and Zingibe officinale L. 0.005% percent of growing bacteria St. aureus (82.22±8.96) (p<0.05); P. mirabilis (94.0±3.08) (p<0.05); C. albicans (62.68±22.18) (p<0.05); E. coli (97.24±2.98) (p