Capillary electrophoresis-contactless conductivity detection compensation techniques in changing environment

High performance, precision, accuracy and robustness separations and detection in changing environment are hardly achieved. Usually, fluctuations of temperature, electromagnetic radiation, mechanical vibration and other factors negatively affect the quality of the data. Using common separation techniques not only the migration, or retention time, but also baseline drift and peak areas are shifted distorting the final results. In electromigration techniques, this is even more visible due to the fact that current density is proportional to the temperature. What is more important, conductivity readings also provide spoiled data during the change of the temperature. Conventional way to deal with this problem is buffering and regulation of the temperature and other changing conditions. Usually, these devices are operated in the laboratory due to large dimension design and inoperable field-portability. In this work an algorithm is proposed for effective real-time data treatment and improvement of quality of the r esults. Applied methodology improved the precision of migration time and reduced baseline shifting effectivelyInstrumentinės analizės APCVytauto Didžiojo universiteta