Reactive extraction and fermental transesterification of rapeseed oil with butanol in diesel fuel media

Simultaneous extraction and transesterification of oil fromrape seeds using a mineral diesel and butanolmixture have been performed to obtain a mineral diesel and biodieselmixture that is currently used as fuel. LipozymeRM IM is an enzyme preparation well known for having major catalytic activity during oil transesterification. The response surfacemethodwas used to optimize the process of simultaneous oil extraction and transesterification. We aimed to not only extract the maximum amount of oil from rape seeds, but also to transesterify the whole amount using butanol. We determined that the optimum conditions for simultaneous oil extraction and transesterification using butanol were as follows: molar butanol to oil ratio — 31:1, amount of ferment preparation Lipozyme RMIM—5.2%, duration—19.6 h, temperature—40 °C, and mineral diesel to oil ratio (w/w)—9:1Vytauto Didžiojo universitetasŽemės ūkio akademij