Genetic diversity of Garganeys (Anas querquedula) revealed with random amplified polymorphic DNA markers

The aim of this study was to define genetic variation within and between Garganeys populations from different East Europe regions (Latvia, Kaliningrad region of Russia, Novgorod region of Russia, and Belarus) and Garganeys wintering in Senegal (Africa). The technique of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied using four random primers (ol-2, ol-9, ol–11, ol-12). Genetic variability was measured for all Garganys investigated and for different populations from various regions. The data of the DNA fragments analysis showed that ol-2, ol-9, ol-11, ol-12 primers fits for investigating the intraspecies genetic variety. Molecular variance (AMOVA) among populations with these primers was 35 % (p ≤ 0.01). Genetic diversity, determinate by individual genetic variability in populations was 65 % (p ≤ 0.01). The mean level of genetic polymorphism was 98 % for all Garganeys (varied from 39 % in Western Belarus population to 64 % in Southern Belarus population). Genetic differentiation was evaluated among four Belarussian populations and between Belarussian and other East European populations: the highest genetic differentiation was detected among both Russian (Kaliningrad and Novgorod) and Latvian populations (FST = 0.537 ± 0.052). The genetic differentiation among four Belarus populations was also high (FST = 0.331 ± 0.057). The genetic structure of all Belarus populations in compare to other East European populations was similar, so the genetic differentiation between these regions was very low (FST = 0.077 ± 0.027). Nei’s genetic variability index for all Garganeys examined was 0.354 (varied from 0.130 in Latvian population to 0.248 in Southern Belarus population). [...]Biologijos katedraVilniaus universiteto Ekologijos institutasVytauto Didžiojo universiteta