Acrylic continuous beds for bioseparation: synthesis and characterization

Continuous beds, also described as monoliths, are the fourth generation of stationary phases. S. Hjertén was the first who published about continuous beds fabrication and application of high-performance liquid chromatography in 1989 [1]. Since then continuous beds have gained a lot of interest, because of its easy preparation and rapid high-efficiency separation. Over the years, there is a trend of miniaturization and for this reason, capillary monolith columns are a great solution. In this work a reviewed how capillary for liquid chromatography is modified using Bind Silane, how during in situ polymerization continuous bed columns are prepared using a solution of acrylic monomers and other additional compounds will be described [2]. Additionally, an evaluation of porosity, hydrodynamic properties and other characteristics of continuous beds will be reviewed [3]Biochemijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta