Evaluation of genetic structure of Impatiens Parviflora populations growing in Lithuania using several types of dominant DNA markers

Online ISSN: 2335-8718Impatiens parviflora is an invasive annual herb which is widespread in many European countries. In Lithuania this species also is considered as successful invader of city parks and forests and is one of the most noxious alien plants. The objective of the study was to estimate genetic diversity of I. parviflora according to several types of dominant DNA markers. Twenty one populations of I. parviflora were sampled to cover all the territory of Lithuania. In total more than 300 individuals were studied, up to 16 plants were sampled in each population. The set of dominant DNA markers (4 ISSR, 8 RAPD primers and 8 AFLP primer combinations) were used for molecular analyses. Agarose gel electrophoresis and capillary electrophoresis (ABI 3130 genetic analyzer) were used for separation and identification of DNA fragments. Comparing the results of obtained data by different dominant markers, the highest polymorphism according to all used markers was assessed in the Central part of Lithuania and the least polymorphic populations were in the West part of the country. Analysis of molecular variance (AMOVA) shown that genetic diversity within populations is lower than among populations according to 3 types of DNA markers. Principal coordinate analyses (PCoA) did not reveal clear differentiation of I. parviflora into separate groups according to geographical location by ISSR and RAPD markers, however AFLP markers shown that populations from West part of Lithuania groups together and populations from East part of the country creates another group, Central Lithuanian populations were more dispersed. Comparing Bayesian clustering analyses, similar numbers of clusters were obtained by separate dominant DNA makers and results suggest that spread of I. parviflora in Lithuania might be determined by complex multiple introduction processBiologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta