Extraction from the medicinal raw material of Glechoma hederacea L. optimization by using spectrophotometric methods for total amount of phenolic compounds and for evaluation of antioxidant activity

Lots of studies with medical raw material are being done because the need of herbal medical products is rapidly increasing. Scientists are trying to find the most efficient technological solutions. There is a great interest in herbal raw antioxidant activity. Antioxidants counteract againts the progression of many chronic diseases. Phenolic compounds are natural antioxidants and they can improve blood circulation, inhibit fat peroxidation and have anti-inflammatory effect. Phenolic compounds are found in plants as secondary metabolites. Glechoma hederacea L. is a potential, not fully known herb what gives an inspiration to work harder with this plant. Besides, this herb is not mentioned in pharmacopoeial articles although there is a huge amount of information that Glechoma hederacea L. could be very useful in pharmacy. Glechoma hederacea L. is a widespread herb of the Nepetoideae subfamily which is also known as ground ivy. It grows widely in Europe, southwestern Asia and has been introduced to North America. Spectrophotometric method enables to detect and evaluate phenolic compounds in Glechoma hederacea L. This method is based on sample solution’s chemicals ability to absorb the light. Extraction optimization must be done to obtain the best results. It allows to find out how to get maximum of phenols. Two types of solvents were used in this study: methanol and ethanol. 0,2 g of five different plant life stages medical raw was filled up with 20 ml of different solvent. After 24 hours of shaking they were filtered and analyzed spectrophotometrically. 100 of a sample was mixed with 3 ml of 20% sodium carbonate solution and 100 of Folin – Ciocalteu reagent (2N). Sample was incubated in room temperature for 30 min and then measured at 760 nm. Quantification was done with respect to the standard calibration curve of rutin. The biggest amount of phenolic compounds was found in butonization phase [...]Biochemijos katedraBotanikos sodasGamtos mokslų fakultetasVytauto Didžiojo universiteta