mesh-induced tissue reaction in a rat model

Objective: To evaluate the effects of menopause, use of steroids, diabetes mellitus, and site of implantation on the tissue response to type I polypropylene mesh used in pelvic reconstructive surgery.Study design: Forty mature female albino rats were used in the study. Inflammatory reaction and meshtissue detachment strength were studied in 4 different animal models; control (GI), menopause (GII), steroid +.menopause (GIII), and diabetes mellitus + menopause (GIV) groups. Two pieces of 1 cm x 1 cm type I macro porous polypropylene monofilament mesh were fixed over rectus abdominis muscle on both sides of the midline, and 0.5 cm x 0.5 cm in size was placed into paravaginal area. Nine weeks later, implanted sling materials in the vaginal region and the right abdominal side were harvested with surrounding tissue for histopathologic examination, whereas the left sided meshes were used for the mechanical testing of detachment strength.Results: The mean detachment strengths in groups were, 595 +/- 274 g for GI, 410 +/- 161 g for GII 610 +/- 202 g for Gill, and 457 +/- 250 g for GIV (p > 0.008). Inflammatory process was more intense in menopause and DM + menopause groups for both abdominal and vaginal tissues (p 0.008). Comparison of tissue reaction caused by meshes in abdominal and vaginal area showed more intense granulocyte infiltration in abdominal region whereas more prominent inflammation and necrosis in the vaginal site (p < 0.05).Conclusion: The abdominal and vaginal region show differences in tissue reaction to type I mesh, and menopause was the most determining factor on the intensity of mesh induced inflammatory response. (C) 2014 Elsevier Ireland Ltd. All rights reserved