Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: Results from the DIVA study group
- Svicher, V.
- Alteri, C.
- Montano, M.
- Nori, A.
- D'Arrigo, R.
- Andreoni, M.
- Angarano, G.
- Antinori, A.
- Antonelli, G.
- Allice, T.
- Bagnarelli, P.
- Baldanti, F.
- Bertoli, A.
- Borderi, M.
- Boeri, E.
- BON, ISABELLA
- Bruzzone, B.
- Barresi, R.
- Calderisi, S.
- Callegaro, A. P.
- Capobianchi, M. R.
- Gargiulo, F.
- Castelli, F.
- Cauda, R.
- Ceccherini Silberstein, F.
- Clementi, M.
- Chirianni, A.
- Colafigli, M.
- D'Arminio Monforte, A.
- De Luca, A.
- Di Biagio, A.
- Di Nicuolo, G.
- Di Perri, G.
- Di Santo, F.
- Fadda, G.
- Galli, M.
- Gennari, W.
- Ghisetti, V.
- Costantini, A.
- Gori, A.
- Gulminetti, R.
- Leoncini, F.
- Maffongelli, G.
- Maggiolo, F.
- Maserati, R.
- Mazzotta, F.
- Meini, G.
- Micheli, V.
- Monno, L.
- Mussini, C.
- Nozza, S.
- Paolucci, S.
- Palù, G.
- Parisi, S.
- Parruti, G.
- Pignataro, A. R.
- Quirino, T.
- RE, MARIA CARLA
- Rizzardini, G.
- Sanguinetti, M.
- Santangelo, R.
- Scaggiante, R.
- Sterrantino, G.
- Turriziani, O.
- Vatteroni, M. L.
- Viscoli, C.
- Vullo, V.
- Zazzi, M.
- Lazzarin, A.
- Perno, C. F.
Publication date
January 2014
DOIAbstract
PURPOSE: We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification. RESULTS: The overall rate of successful V3 sequences ranged from 100 % in samples with >3,000 copies HIV-1 DNA/10(6) PBMCs to 60 % in samples with <100 copies total HIV-1 DNA /10(6) PBMCs. Analys...
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