X-ray fluorescence microscopy of light elements in cells: Self-absorption correction by integration of compositional and morphological measurements
- Malucelli, E
- Iotti, S
- Fratini, M
- Marraccini, C
- Notargiacomo, A
- Gianoncelli, A
- Bukreeva, I
- Cedola, A
- Maier
- J
- Farruggia, G
- Cappadone, C
- Merolle, L
- Wolf
- F
- Trapani, V
- Lagomarsino, S
DOIAbstract
We present here a new methodology for quantitative mapping of light elements in cells, based on combination of compositional and morphological information, derived respectively by X-ray Fluorescence Microscopy (XRFM), Atomic Force Microscopy and Scanning Transmission X-ray Microscopy (STXM). Since XRFM of light elements (carbon, nitrogen, oxygen, sodium and magnesium), are strongly influenced by self-absorption, we developed an algorithm to correct for this effect, using the morphological and structural information provided by AFM and STXM. Finally, the corrected distributions have been obtained, thus allowing quantitative mapping
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