Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical–polyhedral shape cells. The luteinization index (progesterone:17-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes
The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tig...
Oocytes grown in vitro are of low quality and yield few live births, thus limiting the ability to st...
<div><p><i>In vitro</i> growth of follicles is a promising technology to generate large quantities o...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Abstract In vitro ovarian follicle culture is a new frontier in assisted reproductive technology wit...
Infertility in women is a health priority. Designing a robust culture protocol capable of attaining ...
Infertility in women is a health priority. Designing a robust culture protocol capable of attaining ...
The development of techniques allowing the growth of primordial follicles to mature follicles in vit...
The development of techniques allowing the growth of primordial follicles to mature follicles in vit...
Ovarian follicle growth is a prolonged process that involves progressive development of the follicle...
Objective To verify the hypothesis that a three-dimensional, follicle-mimicking structure enhances ...
In vitro culture of ovarian follicles is a new technique in reproductive technology, which helps in ...
Ovarian preantral follicles often flatten and rupture during static culture in a tissue culture well...
The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tig...
Oocytes grown in vitro are of low quality and yield few live births, thus limiting the ability to st...
<div><p><i>In vitro</i> growth of follicles is a promising technology to generate large quantities o...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-lik...
Abstract In vitro ovarian follicle culture is a new frontier in assisted reproductive technology wit...
Infertility in women is a health priority. Designing a robust culture protocol capable of attaining ...
Infertility in women is a health priority. Designing a robust culture protocol capable of attaining ...
The development of techniques allowing the growth of primordial follicles to mature follicles in vit...
The development of techniques allowing the growth of primordial follicles to mature follicles in vit...
Ovarian follicle growth is a prolonged process that involves progressive development of the follicle...
Objective To verify the hypothesis that a three-dimensional, follicle-mimicking structure enhances ...
In vitro culture of ovarian follicles is a new technique in reproductive technology, which helps in ...
Ovarian preantral follicles often flatten and rupture during static culture in a tissue culture well...
The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tig...
Oocytes grown in vitro are of low quality and yield few live births, thus limiting the ability to st...
<div><p><i>In vitro</i> growth of follicles is a promising technology to generate large quantities o...