Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications

In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V. All rights reserved