This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available enzyme-linked fluorescent assay (ELFA) VIDAS ECOLI O157 for detecting Escherichia coli O157 in mincemeat. In addition, a PCR-based survey on Shiga-toxin-producing E. coli (STEC) in mincemeat collected in Italy is presented. Real-time PCR assays targeting the stx genes and a specific STEC O157 sequence (SILO157, a small inserted locus of STEC O157) were tested for their sensitivity on spiked mincemeat samples. After overnight enrichment, the presence of STEC cells could be clearly determined in the 25 g samples containing 10 bacterial cells, while the addition of five bacteria provided equivocal PCR results with Ct values very close to or abo...
Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serio...
Milkborne transmission of Shiga toxin- producing Escherichia coli (STEC) has raised considerable con...
The aim of this study was to monitor the presence of Shiga toxin (Stx)-producing Escherichia coli in...
This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available...
The implementation of direct testing of clinical faecal specimens for gastrointestinal (GI) pathogen...
Molecular diagnostic tools capable of identifying Shiga toxin-specific genetic determinants in stool...
We have developed a rapid (1-h) real-time fluorescence-based PCR assay with the Smart Cycler thermal...
Shigatoxin-producing Escherichia coli (STEC) can cause infections in humans which can beserious and ...
Abstract Background Shiga toxin-producing Escherichia coli (STEC), including E. coli O157:H7, are re...
<p>Shiga toxin-producing Escherichia coli (STEC) remains a major foodborne pathogen of concern...
Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentia...
PCR assays have proved useful for detecting and characterizing Shiga toxin-producing Escherichia col...
The isolation of Shiga toxin-producing Escherichia coli (STEC) other than serogroup O157 from clinic...
The aim of this study was to monitor the presence of Shiga toxin (Stx)-producing Escherichia coli in...
none7Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens that cause out...
Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serio...
Milkborne transmission of Shiga toxin- producing Escherichia coli (STEC) has raised considerable con...
The aim of this study was to monitor the presence of Shiga toxin (Stx)-producing Escherichia coli in...
This work aimed to compare real-time polymerase chain reaction (PCR) with the commercially available...
The implementation of direct testing of clinical faecal specimens for gastrointestinal (GI) pathogen...
Molecular diagnostic tools capable of identifying Shiga toxin-specific genetic determinants in stool...
We have developed a rapid (1-h) real-time fluorescence-based PCR assay with the Smart Cycler thermal...
Shigatoxin-producing Escherichia coli (STEC) can cause infections in humans which can beserious and ...
Abstract Background Shiga toxin-producing Escherichia coli (STEC), including E. coli O157:H7, are re...
<p>Shiga toxin-producing Escherichia coli (STEC) remains a major foodborne pathogen of concern...
Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentia...
PCR assays have proved useful for detecting and characterizing Shiga toxin-producing Escherichia col...
The isolation of Shiga toxin-producing Escherichia coli (STEC) other than serogroup O157 from clinic...
The aim of this study was to monitor the presence of Shiga toxin (Stx)-producing Escherichia coli in...
none7Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens that cause out...
Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serio...
Milkborne transmission of Shiga toxin- producing Escherichia coli (STEC) has raised considerable con...
The aim of this study was to monitor the presence of Shiga toxin (Stx)-producing Escherichia coli in...