Biological properties of solid free form designed ceramic scaffolds with BMP-2: in vitro and in vivo evaluation

Porous ceramic scaffolds are widely studied in the tissue engineering field due to their potential in medical applications as bone substitutes or as bone-filling materials. Solid free form (SFF) fabrication methods allow fabrication of ceramic scaffolds with fully controlled pore architecture, which opens new perspectives in bone tissue regeneration materials. However, little experimentation has been performed about real biological properties and possible applications of SFF designed 3D ceramic scaffolds. Thus, here the biological properties of a specific SFF scaffold are evaluated first, both in vitro and in vivo, and later scaffolds are also implanted in pig maxillary defect, which is a model for a possible application in maxillofacial surgery. In vitro results show good biocompatibility of the scaffolds, promoting cell ingrowth. In vivo results indicate that material on its own conducts surrounding tissue and allow cell ingrowth, thanks to the designed pore size. Additional osteoinductive properties were obtained with BMP-2, which was loaded on scaffolds, and optimal bone formation was observed in pig implantation model. Collectively, data show that SFF scaffolds have real application possibilities for bone tissue engineering purposes, with the main advantage of being fully customizable 3D structures.This work was supported by Ministerio de Ciencia e Innovación (PET2008-0168, CSD2009-00088), and Ministerio de Educación y Ciencia y Fondo Social Europeo (PTQ05-02-02498 and Fundación PCM, MAT 2006-08720). These funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Escherichia-coli-produced recombinant human BMP-2 (rhBMP-2) was generously supplied by Noricum S.L. (Spain). We thank Alba Cuenca-Moya (Noricum S.L.) and Virginia Gutierrez (Pharmakine) for their technical support.S