Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan

The aim of this study was to determine the frequency of ?-globin gene mutations in three groups of Mexican unrelated individuals. The first two groups were normal and sickle cell trait individuals from the Costa Chica region, a place with a 12.8% frequency of HbS carriers, and the third group comprised of Mexican mestizo patients with ?-thalassemia. We searched for -?3.7 and -?4.2 ?+- thalassemia deletion alleles, as well as the ??? anti3.7 triplication through long-gap PCR. The alleles -?3.7 and ???anti3.7 were found in the heterozygote state only; 19% of the normal subjects had the -?3.7 allele, and 2% showed the ??? anti3.7 allele. In individuals with the sickle cell trait, 17% had the -?3.7 deletion, and the ??? anti3.7 triplication was observed in 3% of these individuals. We revealed that 16% of the subjects with ?-thalassemia showed the -?3.7 deletion and 28% the ??? anti3.7 triplication. The -?4.2 deletion was not detected in any individual. The frequency of the -?3.7 allele was roughly the same in the three groups studied; this can be explained by the fact that the three groups have common genes from Africa and the Mediterranean, where a high prevalence of ?+-thalassemia has been observed. To our knowledge, the frequency of ???anti3.7 triplication observed in the Mexican ?-thalassemia patients is the highest reported. As the -?3.7 and ??? anti3.7 alleles are very common in our selected populations, we believe that there is a need to investigate systematically the ?-globin gene mutations in all hemoglobinopathies in the Mexican population. " 2006 Elsevier Inc. All rights reserved.",,,,,,"10.1016/j.bcmd.2005.12.003",,,"http://hdl.handle.net/20.500.12104/43887","http://www.scopus.com/inward/record.url?eid=2-s2.0-33645308826&partnerID=40&md5=f48cd5b1a0418cfd52e53968138df63c",,,,,,"2",,"Blood Cells, Molecules, and Diseases",,"25